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rabbit polyclonal igg antibody raised against cd4  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit polyclonal igg antibody raised against cd4
    Rat pups born to dams fed diets rich in n-3 PUFA (fish oil) have altered colonic T cell balance. Rat dams were fed either 20% fat diets rich in either n-3 or n-6 PUFA and the offspring colons were examined for the presence of T cell markers via immunofluorescence and then quantified on colonic tissues sections. The n-3 rich diets depleted the presence of CD8+ T cells and <t>CD4+</t> Foxp3+ T cells (*, P < 0.05).
    Rabbit Polyclonal Igg Antibody Raised Against Cd4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal igg antibody raised against cd4/product/Santa Cruz Biotechnology
    Average 90 stars, based on 19 article reviews
    rabbit polyclonal igg antibody raised against cd4 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Maternal exposure to fish oil primes offspring to harbor intestinal pathobionts associated with altered immune cell balance"

    Article Title: Maternal exposure to fish oil primes offspring to harbor intestinal pathobionts associated with altered immune cell balance

    Journal: Gut Microbes

    doi: 10.1080/19490976.2014.997610

    Rat pups born to dams fed diets rich in n-3 PUFA (fish oil) have altered colonic T cell balance. Rat dams were fed either 20% fat diets rich in either n-3 or n-6 PUFA and the offspring colons were examined for the presence of T cell markers via immunofluorescence and then quantified on colonic tissues sections. The n-3 rich diets depleted the presence of CD8+ T cells and CD4+ Foxp3+ T cells (*, P < 0.05).
    Figure Legend Snippet: Rat pups born to dams fed diets rich in n-3 PUFA (fish oil) have altered colonic T cell balance. Rat dams were fed either 20% fat diets rich in either n-3 or n-6 PUFA and the offspring colons were examined for the presence of T cell markers via immunofluorescence and then quantified on colonic tissues sections. The n-3 rich diets depleted the presence of CD8+ T cells and CD4+ Foxp3+ T cells (*, P < 0.05).

    Techniques Used: Immunofluorescence



    Similar Products

    rabbit polyclonal igg antibody raised against cd4  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology rabbit polyclonal igg antibody raised against cd4
    Rat pups born to dams fed diets rich in n-3 PUFA (fish oil) have altered colonic T cell balance. Rat dams were fed either 20% fat diets rich in either n-3 or n-6 PUFA and the offspring colons were examined for the presence of T cell markers via immunofluorescence and then quantified on colonic tissues sections. The n-3 rich diets depleted the presence of CD8+ T cells and <t>CD4+</t> Foxp3+ T cells (*, P < 0.05).
    Rabbit Polyclonal Igg Antibody Raised Against Cd4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal igg antibody raised against cd4/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal igg antibody raised against cd4 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    polyclonal rabbit igg against cd4 antibody  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology polyclonal rabbit igg against cd4 antibody
    Sequence of the primer pairs used for reverse transcription-quantitative polymerase chain reaction.
    Polyclonal Rabbit Igg Against Cd4 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit igg against cd4 antibody/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit igg against cd4 antibody - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Rat pups born to dams fed diets rich in n-3 PUFA (fish oil) have altered colonic T cell balance. Rat dams were fed either 20% fat diets rich in either n-3 or n-6 PUFA and the offspring colons were examined for the presence of T cell markers via immunofluorescence and then quantified on colonic tissues sections. The n-3 rich diets depleted the presence of CD8+ T cells and CD4+ Foxp3+ T cells (*, P < 0.05).

    Journal: Gut Microbes

    Article Title: Maternal exposure to fish oil primes offspring to harbor intestinal pathobionts associated with altered immune cell balance

    doi: 10.1080/19490976.2014.997610

    Figure Lengend Snippet: Rat pups born to dams fed diets rich in n-3 PUFA (fish oil) have altered colonic T cell balance. Rat dams were fed either 20% fat diets rich in either n-3 or n-6 PUFA and the offspring colons were examined for the presence of T cell markers via immunofluorescence and then quantified on colonic tissues sections. The n-3 rich diets depleted the presence of CD8+ T cells and CD4+ Foxp3+ T cells (*, P < 0.05).

    Article Snippet: Paraffin-embedded colon sections cut in cross-sections (3 μm) were deparaffinized and antigen retrieval of rehydrated tissues was performed using a 1 mg/mL trypsin (Sigma) followed by incubation with the following primary antibodies: rabbit polyclonal IgG antibody raised against MPO (Thermo Scientific) to examine polymorphonuclear leukocytes; rabbit polyclonal IgG antibody raised against CD163 (Biorbyt) to examine monocytes/macrophages; fluorescein conjugated sheep IgG polyclonal antibody raised against arginase (R&D Systems) to examine M2 macrophages; rabbit monoclonal IgG antibody raised against CD8 (Cedarlane), rabbit polyclonal IgG antibody raised against CD4 (Santa Cruz Biotechnology), rabbit polyclonal IgG antibody raised against CD3 (GeneTex) and rabbit polyclonal IgG antibody raised against Foxp3 (Santa Cruz Biotechnology) were co-incubated with mouse monoclonal IgG2a antibody raised against CD4 (Abcam) to examine populations of T cells.

    Techniques: Immunofluorescence

    Sequence of the primer pairs used for reverse transcription-quantitative polymerase chain reaction.

    Journal: Oncology Letters

    Article Title: FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer

    doi: 10.3892/ol.2016.4439

    Figure Lengend Snippet: Sequence of the primer pairs used for reverse transcription-quantitative polymerase chain reaction.

    Article Snippet: The tissue slides were blocked for 10 min at room temperature (25°C), washed with phosphate-buffered saline (PBS), then incubated with the following anti-human antibodies at room temperature (25°C) for 2 h in moist dark chambers: Monoclonal mouse IgG1 against CD3 (#sc-20047), polyclonal rabbit IgG against CD4 (#sc-7219) and CD8 (#sc-7188; all from Santa Cruz Biotechnology, Inc., Dallas, TX, USA; dilution, 1:100); monoclonal mouse IgG2a against CD45RO (#ab86080; dilution, 1:10), monoclonal rabbit IgG against CEACAM6 (#ab134074; dilution, 1:400) and monoclonal mouse IgG3 against FOXP3 (#ab450; 1:50 dilution; all from Abcam, Cambridge, MA, USA).

    Techniques: Sequencing

    Representative images of CD3, CD4, CD8, CD45RO, CEACAM6 and FOXP3 staining by immunohistochemistry in normal colonic tissue, colonic adenoma and colon cancer. Images show 3,3′-diaminobenzidine tetrahydrochloride hydrate staining of positive cells. CD3, CD4, CD8 and CD45RO were immunolocalized to the membrane, CEACAM6 in the cytoplasmic and FOXP3 in the nucleus. All images were taken at ×400 scanning magnification. CD, cluster of differentiation; CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6; FOXP3, forkhead box P3.

    Journal: Oncology Letters

    Article Title: FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer

    doi: 10.3892/ol.2016.4439

    Figure Lengend Snippet: Representative images of CD3, CD4, CD8, CD45RO, CEACAM6 and FOXP3 staining by immunohistochemistry in normal colonic tissue, colonic adenoma and colon cancer. Images show 3,3′-diaminobenzidine tetrahydrochloride hydrate staining of positive cells. CD3, CD4, CD8 and CD45RO were immunolocalized to the membrane, CEACAM6 in the cytoplasmic and FOXP3 in the nucleus. All images were taken at ×400 scanning magnification. CD, cluster of differentiation; CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6; FOXP3, forkhead box P3.

    Article Snippet: The tissue slides were blocked for 10 min at room temperature (25°C), washed with phosphate-buffered saline (PBS), then incubated with the following anti-human antibodies at room temperature (25°C) for 2 h in moist dark chambers: Monoclonal mouse IgG1 against CD3 (#sc-20047), polyclonal rabbit IgG against CD4 (#sc-7219) and CD8 (#sc-7188; all from Santa Cruz Biotechnology, Inc., Dallas, TX, USA; dilution, 1:100); monoclonal mouse IgG2a against CD45RO (#ab86080; dilution, 1:10), monoclonal rabbit IgG against CEACAM6 (#ab134074; dilution, 1:400) and monoclonal mouse IgG3 against FOXP3 (#ab450; 1:50 dilution; all from Abcam, Cambridge, MA, USA).

    Techniques: Staining, Immunohistochemistry, Membrane

    Immunohistochemical staining scores of T cell subsets in normal colonic tissue, colonic adenoma, stage I–II and stage III–IV cancer tissue. (A) CD3, (B) CD4, (C) CD8, and (D) CD45RO staining scores (n=12 for the normal group, n=38 for the adenoma group, n=40 for the stage I–II group, n=38 for the stage III–IV group). Whiskers represent the range and boxes represent the quartile values. *P<0.05 vs. normal group; ^ P<0.05 vs. adenoma group; # P<0.05 vs. stage I–II cancer group. CD, cluster of differentiation.

    Journal: Oncology Letters

    Article Title: FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer

    doi: 10.3892/ol.2016.4439

    Figure Lengend Snippet: Immunohistochemical staining scores of T cell subsets in normal colonic tissue, colonic adenoma, stage I–II and stage III–IV cancer tissue. (A) CD3, (B) CD4, (C) CD8, and (D) CD45RO staining scores (n=12 for the normal group, n=38 for the adenoma group, n=40 for the stage I–II group, n=38 for the stage III–IV group). Whiskers represent the range and boxes represent the quartile values. *P<0.05 vs. normal group; ^ P<0.05 vs. adenoma group; # P<0.05 vs. stage I–II cancer group. CD, cluster of differentiation.

    Article Snippet: The tissue slides were blocked for 10 min at room temperature (25°C), washed with phosphate-buffered saline (PBS), then incubated with the following anti-human antibodies at room temperature (25°C) for 2 h in moist dark chambers: Monoclonal mouse IgG1 against CD3 (#sc-20047), polyclonal rabbit IgG against CD4 (#sc-7219) and CD8 (#sc-7188; all from Santa Cruz Biotechnology, Inc., Dallas, TX, USA; dilution, 1:100); monoclonal mouse IgG2a against CD45RO (#ab86080; dilution, 1:10), monoclonal rabbit IgG against CEACAM6 (#ab134074; dilution, 1:400) and monoclonal mouse IgG3 against FOXP3 (#ab450; 1:50 dilution; all from Abcam, Cambridge, MA, USA).

    Techniques: Immunohistochemical staining, Staining

    Expression of CD3, CD4, CD8 and CD45RO mRNA in colon tissues of each group. (A) CD3, (B) CD4, (C) CD8 and (D) CD45RO mRNA levels (n=6 for the normal group, n=10 for the adenoma group, n=10 for the stage I–II group, n=9 for the stage III–IV group). *P<0.05 vs. normal group; ^ P<0.05 vs. adenoma group; # P<0.05 vs. stage I–II cancer group. CD, cluster of differentiation.

    Journal: Oncology Letters

    Article Title: FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer

    doi: 10.3892/ol.2016.4439

    Figure Lengend Snippet: Expression of CD3, CD4, CD8 and CD45RO mRNA in colon tissues of each group. (A) CD3, (B) CD4, (C) CD8 and (D) CD45RO mRNA levels (n=6 for the normal group, n=10 for the adenoma group, n=10 for the stage I–II group, n=9 for the stage III–IV group). *P<0.05 vs. normal group; ^ P<0.05 vs. adenoma group; # P<0.05 vs. stage I–II cancer group. CD, cluster of differentiation.

    Article Snippet: The tissue slides were blocked for 10 min at room temperature (25°C), washed with phosphate-buffered saline (PBS), then incubated with the following anti-human antibodies at room temperature (25°C) for 2 h in moist dark chambers: Monoclonal mouse IgG1 against CD3 (#sc-20047), polyclonal rabbit IgG against CD4 (#sc-7219) and CD8 (#sc-7188; all from Santa Cruz Biotechnology, Inc., Dallas, TX, USA; dilution, 1:100); monoclonal mouse IgG2a against CD45RO (#ab86080; dilution, 1:10), monoclonal rabbit IgG against CEACAM6 (#ab134074; dilution, 1:400) and monoclonal mouse IgG3 against FOXP3 (#ab450; 1:50 dilution; all from Abcam, Cambridge, MA, USA).

    Techniques: Expressing